Yang Zhang

Steve Ealick's Research Group

Yang Zhang

PhD: Cornell Univeristy, 2005, Biochemistry

Dissertation:

X-ray Crystallographic and Mechanistic Studies of Three Enzymes

Current Projects:

Structural studies of enzymes involved in the Rut pathway for pyrimidine degradation, in diphthamide biosynthesis, and in PLP biosynthesis, as well as further studies on Toxoplasma adenosine kinase.

Publications:

Chatterjee A, Li Y, Zhang Y, Grove TL, Lee M, Krebs C, Booker SJ, Begley TP, and Ealick SE. Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily. Nat. Chem. Biol. 4, 758-765. Abstract PubMed

Zhang Y, Morar M and Ealick SE. Structural biology of the purine biosynthetic pathway. Cell. Mol. Life Sci.65:3699-3724 (2008). Abstract. PubMed

Zhang Y, White RH and Ealick SE. Crystal Structure and Function of 5-Formaminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5-Monophosphate Synthetase from Methanocaldococcus jannaschii. Biochemistry 47:205-217 (2008). PubMed. (Also includes the structure of the homologous enzyme, as yet functionally uncharacterized, from Pyrococcus furiosus, PfPurP.)

Jenkins AL, Zhang Y, Ealick SE and Begley TP. Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis. Bioorg. Chem. 36:29-32 (2008). PubMed.

Zhang Y and Ealick SE. Purine Nucleoside Phosphorylase in "Computational and Structural Approaches to Drug Discovery Ligand-Protein Interactions", Stroud RM and Finer-Moore J, eds., Royal Society of Chemistry, London (2007). ISBN 0854043659.

Zhang Y, Kang SA, Mukherjee T, Bale S, Crane BR, Begley TP and Ealick SE. Crystal Structure and Mechanism of Tryptophan 2,3-Dioxygenase, a Heme Enzyme Involved in Tryptophan Catabolism and in Quinolinate Biosynthesis. Biochemistry 46:145-155 (2007). PubMed

Zhang Y, Parker WB, Sorscher EJ, and Ealick SE. PNP Anticancer Gene Therapy, Curr. Top. Med. Chem. 5:1259-1274 (2005). PubMed

Zhang Y, Wang W-H, Wu S-W, Ealick SE, and Wang CC. Identification of the Subversive Substrate of Trichomonas vaginalis Purine Nucleoside Phosphorylase and the Crystal Structure of the Enzyme-Substrate Complex. J. Biol. Chem. 280:22318-22325 (2005). PubMed

Zhang Y, Colabroy KL, Begley TP, and Ealick SE. Structural studies on 3-hydroxyanthranilate-3,4-dioxygenase: the catalytic mechanism of a complex oxidation involved in NAD biosynthesis. Biochemistry. 44:7632-7643 (2005). PubMed

Dontsova MV, Gabdoulkhakov AG, Molchan OK, Lashkov AA, Garber MB, Mironov AS, Zhukhlistova NE, Morgunova EYu, Voelter,W, Betzel C, Zhang Y, Ealick SE and Mikhailov AM. Preliminary Investigation of the Three-dimensional Structure of Salmonella typhimurium Uridine Phosphorylase in the Crystal State. Acta Crystallogr. F. 61:337-340 (2005). PubMed

Colabroy KL, Zhai H, Li T, Ying Ge1, Zhang Y, Liu A, Ealick SE, McLafferty FW and Begley TP, The mechanism of inactivation of 3-hydroxyanthranilate-3,4-dioxygenase by 4-chloro-3-hydroxyanthranilate. Biochemistry 44:7623-7631 (2005). PubMed

Zhang Y, Cottet SE and Ealick SE. Structure of E. coli AMP Nucleosidase Reveals Similarity to Nucleoside Phosphorylases. Structure 12: 1383-94 (2004).PubMed

Tolbert WD, Zhang Y, Cottet SE, Bennett EM, Ekstrom JL, Pegg AE and Ealick SE. Mechanism of Human S-Adenosylmethionine Decarboxylase Proenzyme Processing as Revealed by the Structure of the S68A Mutant. Biochemistry 42:2386-2395 (2003). PubMed