Steve Ealick's Research Group
Philmus B, Abdelwahed S, Williams HJ, Fenwick MK, Ealick SE, and Begley TP. Identification of the product of Toxoflavin lyase: degradation via a Baeyer-Villiger oxidation. J. Am. Chem. Soc.134:5326-5330. (2012).
Toxoflavin (an azapteridine) is degraded to a single product by toxoflavin lyase (TflA) in a reaction dependent on reductant, Mn(II), and oxygen. The isolated product was fully characterized by NMR and MS and was iden-tified as a triazine in which the pyrimidine ring was oxidatively degraded. A mechanism for toxoflavin degradation based on the identification of the enzymatic product and the recently determined crystal structure of toxoflavin lyase is proposed.
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