Steve Ealick's Research Group

Recent Publications

Eser BE, Zhang X, Chanani PK, Begley TP, and Ealick SE. From suicide enzyme to catalyst: the iron-dependent sulfide transfer in Methanococcus jannaschii thiamin thiazole biosynthesis. J. Am. Chem. Soc.138:3639-3642 (2016). PubMed Abstract

Zhang X, Eser BE, Chanani PK, Begley TP, and Ealick SE. Structural Basis for Iron-mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases. Biochemistry 55:1826-1838. PubMed Abstract Reports structures of MiThi4, MjThi4, and the C205S mutant of ScThi4.

Mori S, Simkhada D, Zhang H, Erb MS, Zhang Y, Williams HJ, Fedoseyenko D, Russell WK, Kim D, Fleer N, Ealick SE, and Watanabe CM. Polyketide Ring Expansion Mediated by a Thioesterase, CEC Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG. Biochemistry 55:704-714 (2016). Abstract. PubMed

Mehta AP, Abdelwahed SH, Fenwick MK, Hazra AB, Taga ME, Zhang Y, Ealick SE, and Begley TP. Anaerobic 5-Hydroxybenzimidazole formation from aminoimidazole ribotide: an unanticipated intersection of thiamin and vitamin B12 biosynthesis. J. Am. Chem. Soc.137:10444−10447 (2015). Abstract PubMed

Li B, Kim SH, Zhang Y, Hanfrey CC, Elliott KA, Ealick SE, and Michael AJ. Different polyamine pathways from bacteria have replaced eukaryotic spermidine biosynthesis in ciliates Tetrahymena thermophila and Paramecium tetaurelia. Mol. Microbiol. 97:791-807 (2015). Abstract. PubMed

Fenwick MK, Mehta AP, Zhang Y, Abdelwahed SH, Begley TP, and Ealick SE. Noncanonical Active Site Architecture of the Radical SAM Thiamin Pyrimidine Synthase. Nat. Commun. 6:6480 (2015). Abstract. PubMed Reports an additional structure of CcThiC and structures of AtThiC

Mehta AP, Abdelwahed S, Mahanta N, Fedoseyenko D, Philmus B, Cooper L, Liu Y, Jhulki I, Ealick SE, and Begley TP. Radical SAM Enzymes in Cofactor Biosynthesis: a treasure trove of complex organic radical rearrangement reactions. J. Biol. Chem. 290: 3980-3986 (2015). Abstract. PubMed

Ye W, Paul D, Gao L, Seckute J, Sangaiah R, Jayaraj K, Zhang Z, Kaminski PA, Ealick SE, Gold A, and Ball LM. Ethenoguanines Undergo Glycosylation by Nucleoside 2'-Deoxyribosyltransferases at Non-Natural Sites. PLoS One 9:e115082 (2014). Abstract PubMed New structure of Purine 2'-Deoxyribosyltransferase

Sasaki E, Zhang X, Sun HG, Lu MY, Liu TL, Ou A, Li JY, Chen YH, Ealick SE, and Liu HW. Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis. Nature 510:427-431 (2014). Abstract. PubMed (Reports structures of both BexX and BexX-CysO)

Sikowitz MD, Shome B, Zhang Y, Begley TP, and Ealick SE. Structure of a Clostridium botulinum C143S thiaminase I/thiamin complex reveals active site architecture. Biochemistry 52:7830−7839 (2013). Abstract. PubMed

Soriano EV, Zhang Y, Colabroy K, Sanders JM, Settembre EC, Dorrestein PC, Begley TP, and Ealick SE. Active Site Models for Complexes of Quinolinate Synthase with Substrates and Intermediates. Acta Crystallogr. D. 69:1685–1696 (2013). Abstract. PubMed

Sikowitz MD, Cooper LE, Begley TP, Kaminski PA, and Ealick SE. Reversal of Substrate Specificity of CMP N-glycosidase to dCMP. Biochemistry 52:4037–4047 (2013). Abstract PubMed (Reports structures of both Clostridium botulinum BcmB and Saccharomyces rimofaciens MilB)

Hicks KA, O'Leary SE, Begley TP, and Ealick SE. Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae. Biochemistry 52:477-487 (2013). Abstract PubMed

Huang S, Mahanta N, Begley TP, and Ealick SE. Pseudouridine Monophosphate Glycosidase: A New Glycosidase Mechanism. Biochemistry 51:9245-9255 (2012). Abstract. PubMed

Knaus T, Eger E, Koop J, Stipsits S, Kinsland CL, Ealick SE, and Macheroux P. Reverse structural genomics: An unsual flavin binding site in a putative protease from Bacteroides thetaiotaomicron, J. Biol. Chem. 287:27490-27498 (2012). Abstract PubMed

Lai RY, Huang S, Fenwick MK, Hazra AB, Zhang Y, Rajashankar KR, Philmus B, Kinsland C, Sanders JM, Ealick SE, and Begley TP. Thiamin pyrimidine biosynthesis in Candida albicans: a remarkable reaction between histidine and pyridoxal phosphate. J. Am. Chem. Soc. 134:9157−9159. (2012). Abstract. PubMed

Begley TP, Ealick SE, and McLafferty FW. Thiamin biosynthesis: still yielding fascinating biological chemistry. Biochem. Soc. Trans. 40:555-60 (2012). Abstract. PubMed

Dessanti P, Zhang Y, Allegrini S, Tozzi MG, Sgarrella F, and Ealick SE. Structural basis of the substrate specificity of Bacillus cereus adenosine phosphorylase. Acta Crystallogr. D 68:239-248 (2012). Abstract. PubMed. Pdf. Reference

Zhang Y, Zwart PH, and Ealick SE. A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II. Acta Crystallogr. D 68:249-252 (2012). Abstract. PubMed. Pdf. Reference

Philmus B, Abdelwahed S, Williams HJ, Fenwick MK, Ealick SE, and Begley TP. Identification of the product of Toxoflavin lyase: degradation via a Baeyer-Villiger oxidation. J. Am. Chem. Soc. 134:5326-5330. (2012). Abstract PubMed

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