Steve Ealick's Research Group
Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor
PDB file: 1CSG
Colony stimulating factors are glycoproteins that are required for the survival, proliferation, and differentiation of hematopoietic progenitor cells of myeloid and erythroid linkage. Granulocyte-macrophage colony stimulating factor (GM-CSF) stimulates the growth and differentiation of hematopoietic precursor cells from various linearges, including granulocytes, macrophages, erythrocytes, and eosinophils. We have determined the crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) at 2.8 Å resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis.
The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four α-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel β-sheet. In this ribbon drawing, the N terminus is the upper right-hand corner.
Walter MR, Cook WJ, Ealick SE, Nagabhushan, TL, Trotta, PP and Bugg, CE. Three-Dimensional Structure of Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor, J. Mol. Biol. 224:1075-1085 (1992).
Reichert P, Ealick SE, Cook WJ, Trotta P, Nagabhushan TL, Bugg CE. Crystallization and Preliminary X-ray Investigation of Human Granulocyte-Macrophage Colony Stimulating Factor, J. Biol. Chem. 265(1):452-453 (1990)