Steve Ealick's Research Group

Recombinant Human Interferon-γ

PDB file: 1HIG


Interferon-γ is a product of activated T lymphocytes and natural killer cells, which was originally described as an antiviral agent. The x-ray crystal structure of recombinant human interferon-γ has been determined with the use of multiple-isomorphous-replacement techniques and refined to 3.5 Å.

The human interferon-γ protein is primarily α-helical, with six α-helices in each subunit that comprise approximately 62 percent of the structure; there is no β-sheet.

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Interferon-γ, which is dimeric in solution, also crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit.The dimeric structure of human interferon-γ is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions. This view is parallel to the dimer two-fold axis

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Ealick SE, Cook WJ, Senadhi V-K, Carson M, Nagabhushan TL, Trotta PP, and Bugg CE. Three-Dimensional Structure of Recombinant Human Interferon-γ Science 252:698-702 (1991).

Vijay-Kumar S, Ealick SE, Nagabhushan TL, Trotta PP, Kosecki R, Reichert P, and Bugg CE. Crystallization and Preliminary X-ray Investigation of a Recombinant Form of Human Gamma Interferon. J. Biol. Chem, 262:4804-4805 (1987).

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