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Steve Ealick's Research Group |
RNAase A
PDB files:
1YMR, Y92A mutant
1YMW, Y92G mutant
1YMN, Y92L mutant
*2OP2, double-mutant (V43C R85C) with extra disulfide bond
Description:
This work consisted of studying the reductive unfolding pathways of bovine pancreatic ribonuclease. The atomic coordinates of the refined RNase A structure (PDB access code: 1JVU) were used to determine the structure of the mutants by molecular replacement. Analysis of the structures as well as data about thermodynamic, and reductive unfolding properties, shows that a single intramolecular interaction is responsible for the heterogeneity in the reductive unfolding pathways of ribonuclease A.
The crystallographic asymmetric unit consists of one polypeptide chain. |
Reference:
Xu G, Narayan M, Kurinov I, Ripoll DR, Welker E, Khalili M, Ealick SE, and Scheraga HA. A Localized Specific Interaction Alters the Unfolding Pathways of Structural Homologues. J. Am. Chem. Soc. 128:1204-1213 (2006).
Pradeep, L, Kurinov, I, Ealick, SE, and Scheraga, HA. Implementation of a k/k0 Method to Identify Long-Range Structure in Transition States during Conformational Folding/Unfolding of Proteins. Structure 15:1178-89 (2007).