BioMG 7940, Sec 001

Crystallography Made Crystal Clear
Fall 2010

INSTRUCTOR

Prof. Steven E. Ealick
Department of Chemistry and Chemical Biology
382A S. T. Olin Chemistry Research Laboratory

WEB MASTER

Leslie Kinsland

TIME AND PLACE

Tuesday and Thursday, 1:25 - 2:25 PM (note time change)
Room 221 Weill Hall

COURSE DESCRIPTION

The rate of protein structure determination by X-ray crystallography has increased exponentially over the last several decades. Today, the understanding of protein structure contributes to nearly every area of biological research. At the same time, the number of scientists specializing in crystallography has decreased, and in favorable cases the process of structure determination can be automated. Nevertheless, understanding the basics of protein crystallography is important for assessing the strengths and weaknesses of the derived results. The title of this minicourse was borrowed from a textbook by the same name written for nonspecialists (Crystallography Made Crystal Clear, Gale Rhodes, Elsevier, 2006). While the text is not required for this minicourse, I will loosely follow its organization in describing the underlying principles of X-ray crystallography, the experimental techniques employed and the analysis of results.

COURSE SCHEDULE

PowerPoints of the lectures will be posted after each class.

September 21 (Tu): Postponed; made up by extending the class time of subsequent classes
September 23 (Th): An overview of protein crystallography

September 28 (Tu): Protein crystals
September 30 (Th): Collecting diffraction data

October 5 (Tu): Structure Determination
October 7 (Th): Judging the model; Case studies