Chemistry 7880

Macromolecular Crystallography

Spring 2014

Professor Steve Ealick

(see3@cornell.edu; 382 ST Olin)

TTh 1:25 PM - 2:40 PM, Baker 335

3 credits

Prerequisites:

Introductory Calculus
Introductory Physics

Textbook:

Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology by Bernhard Rupp, Garland Science; IBSN #9780815340812.

Course Information

Course Schedule (with links to notes and PowerPoints)

The past decade has seen a revolution in the techniques required for macromolecular crystallography. Crystallization robots are often used for screening conditions and growing crystals, and synchrotron storage rings provide small, stable and intense X-ray beams. These X-ray diffraction experiments are often carried out using sample auto-mounters, and in some cases the experiment can be controlled remotely from the investigator's own office. Software is available for data collection strategy and automated analysis. Once the structure has been determined, software suites such as Phenix are available for refinement. In the most favorable cases, a protein structure can be determined without user intervention. But not all cases are favorable and automated analysis often fails partly or completely. This course will cover the principles of X-ray diffraction in order to answer the question "What do I do when Phenix AutoSol doesn't solve my structure?"